Washington State University

Monitoring

Stream monitoring > Student Projects > BOB

Stream sampling using "bait in a bottle" method at Clarks Creek

Students from Pierce College Biology 213 class collecting water samples at Clarks Creek.

Oomycetes are fungus-like organisms found in marine, freshwater, and terrestrial environments. Some, such as Phytophthora, Pythium, and Saprolegnia, are parasites of plants and animals. DNA sequence data has revealed that these organisms are not fungi, but are more closely related to brown algae and diatoms. We will be “fishing” for these organisms by using leaf baits from various plant species.

Objectives

  • Is there a relationship between host bait material and Oomycete species isolated?
  • Riparian vs forest plants – are riparian plants resistant to infection?
  • Has P. ramorum made it to Clarks Creek?

Each group will choose a plant species to use for bait. Pick fully expanded leaves that are relatively free of spots or other signs of infection. Punch out 20 leaf discs and put in bottle. Include one intact leaf. Put leaf baits in bottle of creek water and cap tightly. Rest bottle on its side for incubation.

After incubation leaf baits will be cultured on selective media for Oomycetes, transferred to pure culture, and identified using DNA sequencing.

Results of sequence analysis

Photos

Water sampling at Clarks Creek

5-3-11

Isolating from leaf baits in the lab

5-10-11

Transferring colonies of Oomycetes to pure culture

5-17-11

PCR for DNA sequence analysis and identification of Oomycetes

5-24-11

Other monitoring at Clarks Creek

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center,2606 West Pioneer, Puyallup, WA, 98371-4998 USA