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Stream monitoring > Student Projects > Noble Fir root inoculation

Inoculation of Noble fir seedlings with Phytophthora and Pythium spp.

For her senior project, a student at Bellarmine High School, Tacoma, is testing several isolates of Phytophthora and Pythium spp. to determine whether they cause disease on Noble fir seedlings. Noble fir (Abies procera) is commonly grown as a Christmas tree in western Washington and some growers irrigate using local stream water. Some of the test organisms have been collected from streams in western WA in the 2011 stream monitoring program. Others are known pathogens of Noble fir and are included as standards or to obtain more information about their behavior on roots.

 

 

 

Test organisms will be selected from this list:

Sample #	Isolate #	Species	Origin
1	1106-BP1-L2A	Pythium undulatum*	Stream bait, North Crk, Snohomish Co.
2	1106-BP1-L4B	Phytophthora taxon “PGchlamydo”*	Stream bait, North Crk, Snohomish Co.
3	109-0045	Phytophthora cryptogea*	Noble fir roots, Puyallup, WA
4	109-0038	Phytophthora gonapodyides*	Noble fir roots, Puyallup, WA
5	1106-BP1-L3B	Phytophthora taxon ‘salixsoil’*	Stream bait, North Crk, Snohomish Co.
6	109-0049	Phytophthora cambivora	Noble fir roots, Puyallup, WA
7	P720	Phytophthora cinnamomi*	Pacific madrone roots, CA
8	1105-BP1-L3A	Pythium sp. #1*	Stream bait, Goldsborough Crk, Mason Co.
9	109-0030	Phytophthora citricola	Noble fir roots, Puyallup, WA
10	109-0055	Phytophthora megasperma*	Noble fir roots, Puyallup, WA
11	1112-BP2-L2B	Pythium anandrum*	Stream bait, Wollochet Crk, Pierce Co.
12	1112-BP1-L2B	Phytophthora inundata*	Stream bait, Wollochet Crk, Pierce Co.
13	1102-BP1-L5B	Pythium grandisporangium	Stream bait, Clarks Crk, Pierce Co.
14	1103-BP3-L4A	Phytophthora taxon "oaksoil"*	Stream bait, Clarks Crk, Pierce Co.

*Used in Trial 1

 

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center,2606 West Pioneer, Puyallup, WA, 98371-4998 USA